The implementation of cut-off values for euploid assignment intends to discount this analytical disturbance, so deviations lower than the established threshold for 2 chromosome copy numbers are reported as euploidy.

Laboratory manipulations and culture conditions may affect euploidy rates as determined by preimplantation genetic testing for aneuploidy (PGT-A); however, it is unknown whether timing of assisted hatching affects PGT-A results.